ruvB [2019-08-08 09:20:26]

Holliday junction DNA helicase, part of the RuvA-RuvB branch migration translocase

Locus: BSU_27730

Isoelectric point: 5.15

Molecular weight: 37.31 kDa

Protein length: 334 aa

Gene length: 1005 bp

Function: DNA repair/ recombination

Product: Holliday junction DNA helicase

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Genetics → DNA repair/ recombination
• Information processing → Genetics → DNA repair/ recombination → Double strand breaks repair

Gene

Coordinates: 2,835,150 2,836,154

Phenotypes of a mutant

• the inactivations of recU and ruvA-ruvB are synthetically lethal PubMed
• the inactivations of recD2 and ruvA-ruvB are synthetically lethal PubMed

The protein

Catalyzed reaction/ biological activity

• RuvB-ATP stimulates RecU-mediated Holliday junction resolution PubMed

Protein family

• ruvB family (single member, according to UniProt)

Structure

• 1IN4 (from Thermotoga maritima, 57% identity) PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: ruvA-ruvB-yrzS-queA-tgt-yrbF
  Description: PubMed

  Regulation

  • the mRNA is processed between tgt and yrbF by RNase Y, this requires the YmcA-YlbF-YaaT complex PubMed

• Genes: ruvA-ruvB
  Description: PubMed

  Regulatory mechanism

  • LexA: repression, PubMed, in LexA regulon

  Regulation

  • induced upon DNA damage (LexA) PubMed

Biological materials

Mutant

• 1A896 (no resistance), PubMed, available at BGSC
• BKE27730 (ruvB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GAGCCGTTCATCCATGAACA, downstream forward: _UP4_CATTTCCAAATGGAGGCTCC
• BKK27730 (ruvB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GAGCCGTTCATCCATGAACA, downstream forward: _UP4_CATTTCCAAATGGAGGCTCC

References

Reviews

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Original publications

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